Human elisa kit alpha interferon instructions
The extensive use of ELISA kits is not only in the laboratory, but many ordinary people will also buy them home for testing. The general use is: human elisa kit, rat elisa kit, mouse elisa reagent Box, fish elisa kit, sheep elisa kit, etc. A simple point is the domestic ELISA kit and the imported ELISA kit. Now let's take a closer look at the next human elisa kit alpha interferon:
Principle: For the detection of alpha interferon levels in human plasma, serum or other human fluid samples. Detection by double antibody sandwich method. The human interferon-α antibody is first made into a solid phase antibody, and then combined with the HRP-labeled α-interferon antibody, and finally TMB color is developed, and the result is judged by color.
step:
1. Dilute the standard solution and dilute the standard solution according to the requirements in the ELISA kit. 2. The blank plate, standard well and sample well are provided on the plate. Add 50 μl of the coated sample to the plate, add 40 μl of the dilution to the sample well and add 10 μl of the sample to be tested. Gently shake evenly. 3. Seal with a sealing film and store at 37 ° C for 30 minutes. 4.30 times the concentrated solution is diluted 30 times with 5.30 minutes with distilled water. The liquid on the plate is removed, washed 5 times, and patted dry. Add the enzyme labeling reagent to each well, except for the blank well 6. Repeat steps 3 and 5
7. Add color developer A to each well and then add developer B. At 37 degrees Celsius, avoid light and shake evenly. 8. Add stop solution, stop the reaction, observe the color 9. Calculate
Principle: For the detection of alpha interferon levels in human plasma, serum or other human fluid samples. Detection by double antibody sandwich method. The human interferon-α antibody is first made into a solid phase antibody, and then combined with the HRP-labeled α-interferon antibody, and finally TMB color is developed, and the result is judged by color.
step:
1. Dilute the standard solution and dilute the standard solution according to the requirements in the ELISA kit. 2. The blank plate, standard well and sample well are provided on the plate. Add 50 μl of the coated sample to the plate, add 40 μl of the dilution to the sample well and add 10 μl of the sample to be tested. Gently shake evenly. 3. Seal with a sealing film and store at 37 ° C for 30 minutes. 4.30 times the concentrated solution is diluted 30 times with 5.30 minutes with distilled water. The liquid on the plate is removed, washed 5 times, and patted dry. Add the enzyme labeling reagent to each well, except for the blank well 6. Repeat steps 3 and 5
7. Add color developer A to each well and then add developer B. At 37 degrees Celsius, avoid light and shake evenly. 8. Add stop solution, stop the reaction, observe the color 9. Calculate
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