Canine Osteopontin (OPN) Quantitative Detection Kit [Kit Name] and Canine Type I
Collagen (COL-1) Quantitative Detection Kit (ELISA) [Kit Use]: This kit is designed for the quantitative detection of canine type I collagen (COL-1) in serum, plasma, and other related liquid samples. [Detection Principle]: The kit utilizes a double-antibody two-step sandwich ELISA method. A pre-coated microplate with polyclonal antibodies specific to canine type I collagen (COL-1) is used. Standards and samples are added to the plate, followed by an incubation period to allow binding. Unbound components are then removed through washing. An enzyme-labeled secondary antibody is added, and after another incubation and wash step, substrate solutions A and B are introduced. The horseradish peroxidase (HRP)-catalyzed reaction converts the TMB substrate into a blue product, which turns yellow upon acidification. The intensity of the color is directly proportional to the concentration of COL-1 in the sample. The optical density (OD) is measured at 450 nm, and the COL-1 concentration is determined by comparing the OD values of the sample with those of the standard curve. This reliable and sensitive method ensures accurate quantification of canine type I collagen in various biological matrices.
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