Commonly used vitamins in cell culture medium and their effects

The biologically active substance that maintains cell growth plays a regulatory and controlling role in cell metabolism. In cell culture, although serum is an important source of vitamins, various media have been added with various vitamins to accommodate more cell line growth.


Fat-soluble vitamins such as: A, D, E, K.


Water-soluble vitamins such as: B1, B2, B6, B12, pantothenic acid, folic acid, biotin, C, nicotinamide, and the like.


Many vitamins are involved in the constituents of the active groups that make up the various enzymes. Without them, the enzyme is inactive and metabolic activities cannot be carried out.


VA is an oligosaccharide-based carrier for the synthesis of glycoproteins in cells, and has an important maintenance effect on epithelial cells. VD is involved in regulating calcium absorption. VE is an antioxidant that prevents oxidation of unsaturated fatty acids in phospholipids that make up biofilms. VK deficiency causes low prothrombin and prolonged clotting time.


Folic acid is an important raw material for the synthesis of tetrahydrofolate, which plays an important role in the biosynthesis of nucleic acids and the biosynthesis of proteins.


Biotin is a component of some specific carboxylases involved in sugar metabolism and fatty acid synthesis.

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