This reagent is intended for research use only. The kit is designed to detect HIV-Gp41 in human serum, plasma, and other related body fluids. The experimental principle of this assay is based on the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. In this technique, a solid-phase antibody, which is a purified HIV-Gp41-specific antibody, is pre-coated onto the microplate wells. When the sample is added, any HIV-Gp41 present in the specimen will bind to the immobilized antibody. After washing to remove unbound substances, an HRP-conjugated anti-HIV-Gp41 antibody is introduced, forming a complex of antibody-antigen-enzyme-labeled antibody. Following another wash step, the substrate TMB is added, which turns blue under the catalytic action of HRP. The reaction is then stopped by adding an acidic solution, causing the color to shift to yellow. The optical density (OD) at 450 nm is measured using a microplate reader, and the result is compared with a predefined cutoff value to determine whether HIV-Gp41 is present in the sample.
The kit includes all necessary components for performing the ELISA. It is available in both 48-well and 96-well configurations. The components include: a sealed bag, 2 pieces of sealing film, a pre-coated enzyme-labeled plate, negative and positive controls (each 0.5 ml per bottle), enzyme standard reagent (3 ml or 6 ml per bottle), sample diluent (3 ml or 6 ml per bottle), developer A and B solutions (3 ml or 6 ml per bottle), a stop solution (3 ml or 6 ml per bottle), and a concentrated wash solution (20 ml × 20 times or 20 ml × 30 times per bottle). All reagents should be stored at 2–8°C. Proper storage is essential to maintain the stability and performance of the kit. Before use, ensure all components are brought to room temperature and mixed thoroughly as instructed. This assay provides a reliable and sensitive method for detecting HIV-Gp41, supporting diagnostic and research applications in virology and immunology.
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