The image titled "Difference between Type A and Type B of standard biosafety cabinets" shows a comparison of various biosafety cabinet models. The experimental setup in the Xinzhuang Instrument Biological Safety Cabinet in Shanghai features specific airflow velocity requirements. For the A1 type, the minimum airflow velocity or average at the front window should be at least 0.38 m/s. Approximately 70% of the air is recirculated through the HEPA filter into the work area, while 30% is exhausted through the vent. In contrast, the A2 model requires a minimum airflow velocity of 0.5 m/s at the front window, with the same 70/30 recirculation ratio. The A2 cabinet creates a negative pressure environment around the contaminated area to prevent any leakage of hazardous substances.
Class B biosafety cabinets are directly connected to an exhaust system. The fan within the cabinet is linked to an emergency power supply, ensuring continuous negative pressure even during power outages, which is crucial for preventing dangerous gas leaks. The minimum airflow velocity for the front window should be at least 0.5 m/s (100 fpm). The B1 type allows 70% of the air to be exhausted via the vent HEPA filter, while 30% is recirculated through the supply port HEPA filter. The B2 model, however, is fully exhausted with no internal air circulation, offering both biochemical and chemical safety controls. It is completely sealed, with operators working through gloves, commonly referred to as glove boxes. Materials enter and exit through a double-door transfer chamber, making it ideal for high-risk biological experiments.
An A2 biosafety cabinet with an external drain pipe cover can be used when limited chemical gas recirculation is allowed. Unlike standard rigid pipes, this cover has an intake hole that draws air from the room, helping maintain airflow balance without disrupting the cabinet’s performance. This setup is suitable only for trace amounts of toxic chemicals. If chemical gas recirculation is not permitted, a B2 model equipped with a rigid exhaust duct must be used. These cabinets require careful installation and maintenance because the built-in fan and external exhaust fan must be properly balanced to ensure operator and product safety.
Shanghai Xinzhuang is a leading manufacturer of laboratory equipment, including solid-phase extraction systems. With advanced technology and a commitment to quality, they offer reliable solutions for scientific research. Contact them for more information.
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