Matters related to degassing of liquid chromatography mobile phase

Related matters of degassing of liquid chromatography mobile phase The mobile phase used in HPLC must be degassed beforehand, otherwise it will easily escape bubbles in the system and affect the operation of the pump. Bubbles also affect column separation efficiency, affecting detector sensitivity, baseline stability, and even undetectable. (The noise increases, the baseline is unstable, and it suddenly jumps). In addition, the oxygen dissolved in the mobile phase may also react with the sample, the mobile phase or even the stationary phase (eg alkylamine). Dissolved gases can also cause changes in the pH of the solvent, causing errors in the separation or analysis results.

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