When we are doing experiments, we often encounter the problem that the elisa kit has low color sensitivity and low sensitivity. The following is a summary of the possible causes and prevention methods of Hengyuan laboratory technicians for new and old customers:
Possible causes and prevention methods:
1. Insufficient incubation time
Fixed clock timing
2. The impact force during washing is too large, the soaking time of the washing liquid is too long, and the washing time is increased.
Reduce washing impact, keep washing time according to the instructions, and accurately remember the number of washings
3. The amount of developer added is insufficient or reversed, or added after mixing.
When the coloring solution is added dropwise, the dropping bottle is vertically downward, the holding force is uniform, and the dropping speed should not be too fast. First, the coloring agent A is added, and the color developing agent B is added, and the A and B liquids cannot be mixed and added.
4. The developer reaction time is insufficient
Accurate timing
5. There is a problem with the quality of distilled water
Determination of the effect of distilled water preparation reagents on enzyme immunoassay
6. The kit is too long in transit and the temperature is too high
The kit is transported in an incubator and a sufficient amount of ice is placed to minimize transit time.
7. Reagents and samples failed to balance before use
Pre-reagent and sample were equilibrated at room temperature for about 30 minutes.
8. Sample and NaN 3 antiseptic, inhibiting enzyme reaction
Sample can not be added with NaN 3 antiseptic
9. The kit expires
Do not use more than the expiration kit
10. The reagent is turned on for too long
Please try to use up in a short time after the reagent is turned on.
11. The pipette is not enough, the pipetting and discharging is too fast, the liquid on the inner wall of the tip is too much or the inner wall is not clean.
Correct the pipette, the tip should be matched, and the tip should be tightly matched each time. Pipetting should not be too fast and emissions should be complete. The inner wall of the tip should be clean and preferably used at one time.
12. The temperature of the incubator is less than 37 °C or the water temperature of the water bath is less than 37 °C)
When placing the reaction plate, pay attention to the temperature of the incubator and adjust it in time. It should not be too much during the incubation period to affect the temperature balance.
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