Human interleukin-35 (IL-35) ELISA test kit instruction manual

**Human Interleukin-35 (IL-35) ELISA Kit Instructions for Use** This kit is intended for research purposes only and is designed to quantify the concentration of human interleukin-35 (IL-35) in various biological samples, including serum, plasma, urine, cell culture supernatants, and other body fluids. **Principle of Operation** The IL-35 ELISA Kit utilizes a sandwich immunoassay technique. A microtiter plate pre-coated with a specific anti-IL-35 antibody is used as the solid phase. After adding the sample, IL-35 binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody then recognizes the captured IL-35, forming an immune complex. Following washing steps, a TMB substrate is added, which changes color in the presence of HRP. The intensity of the color is directly proportional to the amount of IL-35 present in the sample. The reaction is stopped by adding an acidic solution, turning the color from blue to yellow. The absorbance at 450 nm is measured using a microplate reader, and the IL-35 concentration is determined by comparing the sample OD values to a standard curve. **Kit Components** - Microtiter Plate (1 × 48 or 1 × 96 wells) - Standard (90 ng/L) - Standard Diluent - Enzyme Conjugate - Sample Diluent - TMB Substrate A & B - Wash Buffer (concentrated, 20× or 30×) - Sealing Film - Storage Bag **Storage Conditions** All components should be stored at 2–8°C. The kit has a shelf life of 6 months when stored properly. **Sample Preparation Guidelines** - **Serum:** Allow blood to clot at room temperature for 10–20 minutes, then centrifuge at 2000–3000 rpm for 20 minutes. - **Plasma:** Use EDTA or sodium citrate as anticoagulant, mix well, and centrifuge similarly. - **Urine:** Centrifuge at 2000–3000 rpm for 20 minutes after collection. - **Cell Culture Supernatant:** Centrifuge at 2000–3000 rpm for 20 minutes. For intracellular components, lyse cells via repeated freeze-thaw cycles before centrifugation. - **Tissue Samples:** Homogenize in PBS, centrifuge, and collect the supernatant. **Important Notes** - Avoid repeated freezing and thawing of samples. - Do not use samples containing NaN3, as it may inhibit HRP activity. - Always prepare a standard curve with duplicate wells. If the sample OD exceeds that of the highest standard, dilute the sample appropriately and adjust calculations accordingly. - Ensure all reagents are brought to room temperature before use. - Discard any unused enzyme conjugate after opening. **Procedure Summary** 1. Prepare standards by serial dilution. 2. Add samples and standards to the microplate. 3. Incubate at 37°C for 30 minutes. 4. Wash the plate 5 times with diluted wash buffer. 5. Add HRP-labeled antibody and incubate again. 6. Wash the plate thoroughly. 7. Add TMB substrate and incubate for 15 minutes. 8. Stop the reaction with stop solution. 9. Measure OD at 450 nm within 15 minutes. 10. Calculate concentrations using the standard curve. **Performance Characteristics** - Linear range: 3 ng/L – 70 ng/L - Correlation coefficient (R²): ≥ 0.990 - Intra-assay and inter-assay variability: <9% and <11%, respectively **Safety and Disposal** All waste materials should be handled as biohazardous. Follow local regulations for disposal. **Note:** This manual is for reference only. Always refer to the latest version provided by the manufacturer. (Word count: 578)

Kraft Paper Bowl

1, kraft paper food containers can effectively protect food. It has good moisture resistance and can prevent food from being spoilt by moisture. At the same time, kraft paper has good permeability, which can maintain the freshness and taste of food.

2. Brown paper container is a degradable material, friendly to the environment. Compared with plastic containers, kraft paper containers are easier to decompose in the natural environment and will not pollute the soil and water. Moreover, kraft paper is a renewable resource that can be continuously produced through plant regeneration.

3, kraft containers also have good heat resistance. It can withstand high temperatures and is suitable for the packaging of hot food, such as hot soup, hot pasta and so on. This makes kraft paper containers widely used in the fast food and take-out industries.

Kraft paper food containers are an excellent packaging option. It can protect the quality and taste of food, and reduce the negative impact on the environment. In the pursuit of sustainable development today, the use of kraft paper food containers can contribute to the food industry and promote the popularity of environmental protection awareness.

Kraft Paper Bowl,Paper Kraft Bowl,Kraft Bowls ,Paper Bowl Kraft

EVER GREEN(ZHEJIANG)NEW MATERIAL CO., LTD / KUNSHAN GREENPACK CO.,LTD , https://www.changjingpack.com