This kit is intended for research purposes only. It is designed for the quantitative detection of danofloxacin residues in various biological and environmental samples, including feed, fish, shrimp, meat tissues (such as chicken, beef, and pork), serum, and urine. The method employed is a competitive ELISA, which allows for accurate and sensitive measurement of danofloxacin levels.
The experimental principle is based on a competitive binding reaction. A microplate is pre-coated with danofloxacin antigen. When the sample or standard solution is added, free danofloxacin in the sample competes with the danofloxacin-conjugated antigen for binding sites on the antibody. After incubation, the enzyme-labeled antibody is introduced, followed by the addition of TMB substrate. The reaction produces a blue color, which changes to yellow upon the addition of the stop solution.
The absorbance is measured at 450 nm using a microplate reader. The intensity of the color is inversely proportional to the concentration of danofloxacin in the sample. By constructing a standard curve using known concentrations of danofloxacin, the exact level of the compound in the test sample can be accurately determined. This method provides a reliable and efficient way to monitor danofloxacin residues in different matrices, ensuring compliance with safety and quality standards in food and veterinary applications.
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